Using metabolomics to study the dynamics of metabolism

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27 February 2024

Dysregulation of metabolism is closely linked to the development of various diseases and lifetime changes, including metabolic disorders, cancer, and ageing. Understanding the nuances of cellular metabolism is therefore crucial for elucidating disease mechanisms and identifying potential therapeutic targets. In our laboratory, we are driven by a strong motivation to unravel the mysteries of metabolism and its impact on human health.

Mitochondrial function

The mitochondria are often referred as the powerhouse of the cell. These organelles are essential for energy production through oxidative phosphorylation, generating adenosine triphosphate (ATP) - the primary energy currency of the cell. Beyond ATP synthesis, mitochondria are intricately involved in numerous metabolic processes, including tricarboxylic acid cycle (TCA) cycle, fatty acid oxidation, amino acid metabolism. Drugs may induce changes in mitochondrial function, and thereby alter cellular respiration and metabolism.

Metabolomics Workflow

We employ advanced techniques such as metabolomics utilizing liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) spectroscopy to characterise and monitor metabolites in human cell systems. Metabolomics workflows are developed to monitor metabolite concentrations, assist in metabolite identification, and allow the study of pathway turnover using stable isotope tracers.

By delving into the complex interplay of metabolites within their cellular environment, we aim to uncover novel insights into the mechanistic of metabolic reactions, pathways, and sub-networks. This will allow us to develop targeted therapeutic strategies, mitigate drug toxicity through bioactivation, contribute to combating metabolic disorders and thereby improve human health.

For more information about our research, contact [email protected]